Identification of Ruscus steroidal saponins by HPLC-MS analysis
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Published on: November 11, 2022
E de Combarieu1, M Falzoni, N Fuzzati
1Indena SpA, Research and Development Laboratories, Via Don Minzoni 6, 20090 Settala (MI), Italy.
Abstract:
A novel HPLC-UV method has been developed for the fingerprint analysis of the steroidal saponins in the rhizomes of three Ruscus species (Ruscus aculeatus, Ruscus hypoglossum and Ruscus colchicus). Saponins were identified by HPLC-ESI-MS. During the study a new major saponin was detected in the rhizomes of R. hypoglossum and R. colchicus. The structure of the new compound was defined as 1-O-[alpha-L-rhamnopyranosyl-(1-->2)-6-O-acetyl-beta-D-galactopyranosyl]-1beta,3beta,22xi,26-tetrahydroxy-furost-5(6)-en-26-O-beta-D-glucopyranoside (8) by spectral analysis.