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Updated: Sep 28, 2026

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes
Published on: January 27, 2023
CD14 and TNfa promoter polymorphisms in patients with acute arthritis. Special reference to development of chronic
Heikki Repo1, Krista Anttonen, Sami K Kilpinen
1Department of Medicine, Division of Infectious Diseases, Helsinki University Central Hospital, Haartman Institute, University of Helsinki, Finland. heikki.repo@hus.fi
Objective:
To examine CD14 and TNFalpha gene polymorphisms in early arthritis in relation to clinical outcome.
Methods:
We studied 141 Caucasians who had had early arthritis 10 to 38 years earlier. We analysed CD14 (-159) and TNFalpha (-238, -308, -376) polymorphisms using a novel cycle minisequencing method. DNA pools from 370 Caucasian blood donors served as controls.
Results:
CD14 (-159)C-->T allele frequencies were comparable among patients and controls (39% vs 40%). Fifty men and 42 women had recovered while 24 men and six women had chronic spondyloarthropathy (SpA). Mutant T allele frequency was higher in the chronic SpA group than in the recovered group in women (75% vs 32%, relative risk 1.3, 95% confidence limit 1.1 to 1.6, P = 0.011), but not in men (38% vs 44%). All female patients with chronic SpA had CD14 (-159)T allele and none had a possibly protective TNFalpha (-308)G-->A allele.
Conclusions:
Possession of CD14 (-159)T allele does not increase risk of ReA but may increase susceptibility of female patients to development of chronic SpA.
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