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Related Experiment Videos

Dialyzable free cortisol after stimulation with Synacthen.

Michael Vogeser1, Josef Briegel, Reinhart Zachoval

  • 1Institute of Clinical Chemistry Ludwig-Maximilians-Universität Munich, Klinikum Grosshadern, 81366 Munich, Germany. Michael.Vogeser@klch.med.uni-muenchen.de

Clinical Biochemistry
|December 21, 2002
PubMed
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Acute stimulation of the adrenal cortex significantly increases free cortisol more than total cortisol. This finding highlights the greater biological activity of free cortisol after stimulation.

Area of Science:

  • Endocrinology
  • Biochemistry
  • Pharmacology

Background:

  • Cortisol is a vital hormone regulating metabolism and stress response.
  • Serum cortisol exists in free and bound forms, with free cortisol being the biologically active component.
  • Understanding the dynamics of free versus total cortisol is crucial for accurate assessment of adrenal function.

Purpose of the Study:

  • To compare the changes in free versus total serum cortisol concentrations following acute stimulation of the adrenal cortex.
  • To investigate the impact of adrenocorticotropic hormone (ACTH) stimulation on the ratio of free to total cortisol.

Main Methods:

  • Paired serum samples were collected from ten individuals before and 1 hour after intravenous ACTH (1-24) administration.
  • Total cortisol was quantified using liquid chromatography-tandem mass spectrometry.

Related Experiment Videos

  • Free cortisol was measured after equilibrium dialysis, and corticosteroid-binding globulin (CBG) was determined by radioimmunoassay.

    • Main Results:

    • Total serum cortisol increased by 106% post-stimulation (p < 0.001).
    • Free serum cortisol showed a substantially greater increase of 263% (p < 0.001).
    • The ratio of free to total cortisol significantly increased from 6.1% to 10.6% (p < 0.001), with no significant change in CBG levels.

    Conclusions:

    • Acute neuroendocrine stimulation of the adrenal cortex leads to a more pronounced relative increase in free, bioactive cortisol concentrations compared to total cortisol.
    • The enhanced increase in free cortisol suggests a greater contribution to the physiological response after stimulation.