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Protein Membrane Overlay Assay: A Protocol to Test Interaction Between Soluble and Insoluble Proteins in vitro
08:38

Protein Membrane Overlay Assay: A Protocol to Test Interaction Between Soluble and Insoluble Proteins in vitro

Published on: August 15, 2011

A solid-state competitive binding radioimmunoassay for measurement of antigens solubilized from membranes

M Prat, P M Comoglio

    Journal of Immunological Methods
    |January 1, 1976
    PubMed

    Abstract:

    A sensitive, solid-state, competitive radio immunological method for quantitation of antigens solubilized from membranes is described. This method is based on the measurement of the displacing activity of unknown samples of antigens on 125I-labelled antibodies bound to antigen-coated polyvinyl microtitre wells.

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