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Related Experiment Videos

Voltage-addressable on/off microvalves for high-pressure microchip separations.

Brian J Kirby1, Timothy J Shepodd, Ernest F Hasselbrink

  • 1Microfluidics Department, Sandia National Laboratories, P.O. Box 969, MS 9951, Livermore, CA, USA. bjkirby@sandia.gov

Journal of Chromatography. A
|December 25, 2002
PubMed
Summary

We developed a microchip valve for high-pressure liquid chromatography (HPLC) using laser-patterned polymers. This robust design handles high pressures and enables multiple separations on a single chip.

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Area of Science:

  • Analytical Chemistry
  • Microfluidics
  • Separation Science

Background:

  • High-pressure liquid chromatography (HPLC) is a critical analytical technique.
  • Microfluidic devices offer potential for miniaturized and integrated separation systems.
  • Existing microfluidic valves often struggle with the high pressures and solvent compatibility required for HPLC.

Purpose of the Study:

  • To develop a novel microchip-based valve architecture compatible with HPLC.
  • To enable precise fluid control within microfluidic systems for chromatographic separations.
  • To demonstrate the potential for multiplexing multiple chromatographic analyses on a single microchip.

Main Methods:

  • Fabrication of microchannels in glass substrates.
  • Laser photopatterning of polymer monoliths to create mobile fluid control elements.

Related Experiment Videos

  • Utilizing electrokinetic pressures for valve actuation (opening and closing).
  • Testing valve performance in water-acetonitrile mixtures at pressures up to 350 bar.

    • Main Results:

    • Demonstrated a voltage-addressable on/off valve architecture.
    • Achieved high pressure resistance (up to 350 bar) in HPLC-compatible solvents.
    • Obtained high open/closed flow ratios (10^4 to 10^6) across a significant pressure range (1.5-70 bar).
    • Showcased the potential for precise flow control in packed or monolithic chromatography columns.

    Conclusions:

    • The developed microchip valve platform is robust and compatible with HPLC conditions.
    • This technology facilitates the integration and multiplexing of multiple chromatographic separations on a single microchip.
    • The valve architecture offers a promising solution for advanced microfluidic-based analytical systems.