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Updated: Sep 28, 2026

Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay
Published on: June 9, 2011
First Isolation of West Nile virus from a patient with encephalitis in the United States
Cinnia Huang1, Brett Slater, Robert Rudd
1Wadsworth Center, New York State Department of Health, Albany 12201, USA. Cinnia.huang@wadsworth.org
Abstract:
West Nile virus (WNV) was isolated from a patient who developed encephalitis while undergoing treatment with CHOP (cyclophosphamide, hydroxydoxorubicin, vincristine [Oncovin], predisone) and rituximab for a non-Hodgkin B-cell lymphoma. Both standard reverse transcription-polymerase chain reaction (RT-PCR) and Taqman RT-PCR established the diagnosis of WNV infection from cerebrospinal fluid (CSF). Several whole blood samples and one serum sample underwent further testing. CSF and serum samples were negative for WNV antibody; however, all samples were positive by both RT-PCR assays. Infectious virus was recovered from a blood sample, and its identity was confirmed by using a WNV-specific immunofluorescence assay. The complete WNV genomes determined from CSF and from the virus isolate adapted from cell culture were the same. The results represent the first complete WNV genome sequence obtained directly from human CSF and the first time that infectious WNV has been recovered from a patient with encephalitis in North America.
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