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Related Experiment Videos

Ceramide-induced alterations in dopamine transporter function.

Evan L Riddle1, Kristi S Rau, Matthew K Topham

  • 1Department of Pharmacology and Toxicology, University of Utah, Room 201, 30 South 2000 East, Salt Lake City, UT 84112, USA.

European Journal of Pharmacology
|December 25, 2002
PubMed
Summary
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Ceramide affects dopamine and serotonin transporters. It decreases dopamine uptake while increasing serotonin uptake via the dopamine transporter, revealing ceramide as a novel monoamine transporter modulator.

Area of Science:

  • Neuroscience
  • Biochemistry
  • Pharmacology

Background:

  • Ceramides are bioactive lipids involved in various cellular processes.
  • Monoamine transporters, such as dopamine and serotonin transporters, are crucial for neurotransmission.
  • Dysregulation of these transporters is implicated in neurological and psychiatric disorders.

Purpose of the Study:

  • To investigate the impact of ceramide on dopamine and serotonin transporters.
  • To elucidate the mechanism by which ceramide influences monoamine uptake.

Main Methods:

  • Utilized rat striatal synaptosomes to assess dopamine and serotonin uptake.
  • Employed C2-ceramide, a synthetic ceramide analog.
  • Conducted experiments using selective dopamine transporter inhibitors and substrate competition assays.

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Main Results:

  • C2-ceramide caused a concentration-dependent decrease in dopamine uptake.
  • Ceramide exposure led to an increase in serotonin uptake in striatal synaptosomes.
  • Evidence suggests serotonin uptake occurred via the dopamine transporter, not the serotonin transporter.

Conclusions:

  • Ceramide acts as a novel modulator of monoamine transporter function.
  • Ceramide may alter the affinity of dopamine transporters for dopamine.
  • These findings suggest a potential role for ceramides in regulating neurotransmitter systems.