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The pH modulator chloroquine blocks trypsinogen activation peptide generation in cerulein-induced pancreatitis
Yasuji Seyama1, Taiichi Otani, Akira Matsukura
1Hepato-Biliary-Pancreatic Surgery Division, Department of Surgery, University of Tokyo, Tokyo, Japan.
Introduction And Aims:
We examined the effects of a weak base, chloroquine, on the trypsinogen processing in cerulein-induced pancreatitis.
Methodology:
Immunofluorescence studies were performed using newly generated affinity-purified antibodies to the trypsinogen activation peptide (TAP).
Results:
The present study showed that chloroquine pretreatment blocked intracellular TAP generation in cerulein-induced pancreatitis.
Conclusion:
These results indicate that intracellular trypsinogen activation, which plays an important role in acute pancreatitis, requires a low-pH compartment, as well as serine protease activity.