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Related Experiment Videos

Characterization of cell types during rat liver development.

Henning C Fiegel1, Jonas J h Park, Michael V Lioznov

  • 1Department of Pediatric Surgery, University Hospital Eppendorf, Hamburg, Germany. fiegel@uke.uni-hamburg.de

Hepatology (Baltimore, Md.)
|December 25, 2002
PubMed
Summary

Researchers identified fetal liver stem cell candidates by examining Thy1 and CK-18 markers during rat liver development. Thy1 and CK-18 co-expression suggests distinct hepatic progenitor cell populations exist.

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Area of Science:

  • Hepatology
  • Developmental Biology
  • Stem Cell Research

Background:

  • Hepatic stem cells are present in adult livers.
  • Bone marrow has been identified as a source for hepatic progenitors and hepatocytes.
  • Hematopoietic and hepatic stem cells share markers like CD34, c-kit, and Thy1.

Purpose of the Study:

  • Investigate the stem cell marker Thy1 and hepatocytic marker CK-18 during rat liver development.
  • Identify potential fetal liver stem cell candidates.

Main Methods:

  • Harvested rat fetal livers at various developmental stages (ED 16-22).
  • Purified cells using collagenase-DNAse digestion and Percoll centrifugation.
  • Utilized magnetic cell sorting (MACS) with OX43 and OX44 antibodies.

Related Experiment Videos

  • Characterized cells via immunocytochemistry for Thy1, CK-18, Ki-67, and double labeling for Thy1/CK-18.

    • Main Results:

    • Thy1 expression was observed throughout all liver development stages.
    • Thy1-positive cells were enriched post-MACS in early developmental stages.
    • CK-18-positive cells showed enrichment after MACS across all developmental stages.
    • Cells co-expressing Thy1 and CK-18 were identified, indicating distinct progenitor populations.

    Conclusions:

    • Hepatic progenitor cells (CK-18 positive) in fetal rat liver express Thy1.
    • The study indicates the coexistence of different hepatic cell compartments.
    • Further isolation and characterization are necessary to confirm the biological properties of these cells.