Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Variable tandem repeat VcA of Vibrio cholerae].

S O Vodop'ianov1, I P Oleĭnikov, E K Goncharov

  • 1Institute of Antiplague Research, Rostov-on-Don, 344007 Russia. vodopyanov_s@yahoo.com

Molekuliarnaia Biologiia
|December 26, 2002
PubMed
Summary

Researchers identified a novel genetic marker, VcA, within the Vibrio cholerae genome. This variable-number tandem-repeat (VNTR) locus can differentiate between various strains of V. cholerae.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[The Effectiveness of STR Genotyping Depends on the Organic Carbon Content in Ancient Samples].

Molekuliarnaia biologiia·2026
Same author

[A case of pleuropulmonary dirofilariasis caused by Dirofilaria repens. Case report].

Terapevticheskii arkhiv·2023
Same author

[DNA Phenotyping of Remains from Elite Burials of the Khazar Period of Southern Russia].

Molekuliarnaia biologiia·2023
Same author

[Inguinal lymphadenitis due to invasion of Dirofilaria repens].

Terapevticheskii arkhiv·2021
Same author

[Structural and Functional Organization of the Mitochondrial DNA Control Region in the Woolly Mammoth (Mammuthus primigenius)].

Molekuliarnaia biologiia·2019
Same author

[Termination of Replication and Mechanisms of Heteroplasmy in Sturgeon Mitochondrial DNA].

Molekuliarnaia biologiia·2019

Area of Science:

  • Microbiology
  • Genomics
  • Molecular Biology

Background:

  • Vibrio cholerae is a significant human pathogen responsible for cholera outbreaks.
  • Accurate molecular typing of V. cholerae strains is crucial for epidemiological surveillance and control.
  • Variable-number tandem repeats (VNTRs) are powerful genetic markers for microbial typing.

Purpose of the Study:

  • To identify and characterize novel VNTR loci in the Vibrio cholerae genome.
  • To evaluate the potential of identified VNTR loci as molecular markers for V. cholerae strain typing.

Main Methods:

  • Bioinformatic analysis of the V. cholerae genome to identify potential VNTR loci.
  • Primer design for amplification of selected VNTR loci.
  • PCR-based amplification and analysis of the VcA locus across a collection of V. cholerae strains.

Related Experiment Videos

Main Results:

  • Seven potential VNTR loci were identified in the V. cholerae genome.
  • The VcA locus, characterized by a TGCTGT repeat on chromosome 2, was present in all tested strains.
  • The VcA locus exhibited significant allelic diversity, with repeat numbers ranging from 3 to 23, resulting in 14 observed alleles.

Conclusions:

  • The VcA locus is a highly polymorphic and universally distributed marker in Vibrio cholerae.
  • The VcA locus demonstrates significant potential as a reliable marker for the molecular typing and epidemiological analysis of V. cholerae strains.