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Epoxide hydrase activity in mouse skin eidermis.

W G Pyerin, E Hecker

    Zeitschrift Fur Krebsforschung Und Klinische Onkologie. Cancer Research and Clinical Oncology
    |January 1, 1975
    PubMed
    Summary
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    Researchers developed a new method to detect epoxide hydrolase (EH) activity in mouse epidermis. This technique enhances sensitivity, allowing for clear identification of EH in skin tissue.

    Area of Science:

    • Biochemistry
    • Dermatology
    • Toxicology

    Background:

    • Epoxide hydrolase (EH) plays a crucial role in metabolizing epoxides, which are reactive compounds found in the environment.
    • Measuring EH activity in tissues like the epidermis is challenging due to low endogenous levels.
    • Existing radioassays are often not sensitive enough for low-activity tissues.

    Purpose of the Study:

    • To develop a highly sensitive method for detecting epoxide hydrolase (EH) activity in mouse epidermis.
    • To enable unambiguous demonstration of EH presence and activity in skin tissue.

    Main Methods:

    • Fractionation of mouse epidermis to isolate a "microsomal" fraction.
    • Utilizing a radioassay with 7-3H-styrene oxide as a substrate.
    • Employing an enrichment strategy to increase specific EH-activity.

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    Main Results:

    • A "microsomal" fraction from mouse epidermis showed significantly higher EH-activity (6-7 times) compared to homogenates.
    • The enrichment procedure made the radioassay sufficiently sensitive to detect EH activity in the epidermis.
    • Unambiguous evidence of EH activity in mouse skin epidermis was successfully obtained.

    Conclusions:

    • A refined fractionation and radioassay method allows for sensitive detection of epoxide hydrolase activity in mouse epidermis.
    • This advancement facilitates further research into the role of EH in skin biology and toxicology.
    • The developed technique can be applied to study EH in other tissues with low enzyme levels.