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Partial characterization of a 36-kDa protein from Clostridium botulinum type E that inhibits trypsin and chymotrypsin
Sakthivel Prabakaran1, Bibhuti R. DasGupta
1Department of Food Microbiology and Toxicology, University of Wisconsin, 1925 Willow Drive, Madison, WI 53706, USA.
The Journal of General and Applied Microbiology
|December 27, 2002
Abstract:
A 36-kDa trypsin inhibitor was purified from Clostridium botulinum type E culture supernatant by multiple molecular sieve and ion exchange chromatographic steps. The sequence of the amino-terminal 13 amino acid residues of this single-chain protein is Asn.Gln.Glu.Val.Phe.Asn.Met.Pro.Lys.Phe.Ser.Thr.Ala-. This novel protein that also inhibits chymotrypsin is produced by an organism that does not appear to produce any protease.