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Restriction analysis of the ITS region for characterization of the Debaryomyces species

Jesus P. Ramos1, Patricia Valente, Allen N. Hagler

  • 1Departamento de Genética, Instituto de Biologia, CCS, bloco A, Prof. Paulo de Goes, Universidade Federal do Rio de Janeiro, Ilha do Fundao, Rio de Janeiro, CEP: 21944-970, Brasil.

The Journal of General and Applied Microbiology
|December 27, 2002
PubMed

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IR spectra are divided into two main regions: the diagnostic region and the fingerprint region. The diagnostic region of the spectrum lies above 1500 cm−1. The absorptions resulting from single-bond vibrations of the N–H, C–H, and O–H stretch at higher wavenumbers and appear on the left side of the spectrum. The stretching absorptions of the C≡C and C≡N occur between 2100–2300 cm−1. In contrast, those arising from stretching absorptions of the C=O, C=N, and C=C occur between 1600–1850 cm−1.
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