Related Experiment Video
Updated: Sep 28, 2026

Preparation of High-Quality Fermented Fish Product
Published on: August 23, 2019
Comparative studies on some quality attributes of firm tofu sterilized with traditional and autoclaving methods
Tzou-Chi Huang1, Hui-Yin Fu, Chi-Tang Ho
1Department of Food Science, National Pingtung University of Science and Technology, Taiwan. tchuang@mail.npust.edu.tw
Abstract:
An innovative retort cooking method was developed for firm tofu sterilization to replace the traditional hydrogen peroxide treatment. The caramelized firm tofu was sterilized by autoclaving at 105 degrees C for 20 min, which led to a 3 log cycle reduction of total plate count. Shelf life of the processed firm tofu was extended at least 3 months. As compared with chemical treatment, thermal treatment degraded total and individual isoflavones to a lesser extent, and only minor changes were observed. The percent DPPH free radical scavenging capacity of autoclaved firm tofu was significantly higher than that of the hydrogen peroxide treated samples. Even after prolonged heat treatment, the three-dimensional network structure of the autoclaved firm tofu did not change significantly. Texture profile analysis and sensory evaluation confirmed autoclaved firm tofu to be acceptable.
More Related Videos
10:00Isolation of Tomato Seed-Associated Microbiota Through Controlled Germination and Comparative Culturing
Published on: July 7, 2026
10:03Investigating the Detrimental Effects of Low Pressure Plasma Sterilization on the Survival of Bacillus subtilis Spores Using Live Cell Microscopy
Published on: November 30, 2017
Related Concept Videos
Methods of Sterilization I: Physical Methods
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
Physical Methods for Controlling Microbial Growth: Temperature
Pasteurization and Food Preservation
Methods of Sterilization II: Chemical Methods
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
Methods of Controlling Food Spoilage
Key Techniques in Microbiology