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Published on: May 27, 2011
[Antitumor immunity elicited by 4-1BBL gene transfected Hepa1-6 in vivo]
Xiaohong Li1, Xiaodong Li, Jianyong Huang
1Shanghai Entry-Exit Inspection and Quarantine Bureau, Technical Center for Animal, Plant and Food Inspection and Quarantine, Shanghai 200135, China.
Objective:
To study the effects of 4-1BBL on antitumor immunity induced in vivo by murine 4-1BBL gene transfected Hepa1-6.
Methods:
Retrovirus vector was used to transfer the 4-1BBL gene into syngeneic murine heptocellular carcinoma cell line Hepa1-6. The products were termed as Hepa1-6/4-1BBL, and then the TCV4-1BBL was obtained by treating them with mitomycin (MMC). Three models (immunological model, early model, and later model) were established to study the antitumor effects of TCV4-1BBL.
Results:
(1)In immunological models, the syngeneic mice were completely protected by inoculation with TCV4-1BBL, survived free from tumor for a long period (over 100 days). (2)In early models (7 days after inoculation), Hepa1-6 tumor cells showed strong immunogenicity effects and (3) In later models (14 days after inoculation), they had obvious antitumor effects and most of the tumors were disappeared.
Conclusions:
The antitumor effect against syngeneic murine hepatocellular carcinoma in vivo is obviously enhanced by treating them with TCV4-1BBL
