Related Experiment Videos
A new method to enhance contrast of ultrathin cryosections for immunoelectron microscopy
Toshihiro Takizawa1, Clark L Anderson, John M Robinson
1Department of Physiology, Ohio State University, Columbus, Ohio 43210, USA. takizawa.2@osu.edu
Abstract:
Adequate contrast of ultrathin cryosections is crucial for evaluating morphological detail to assess immunocytochemical localization at the electron microscopic level. We have developed a positive staining method for achieving contrast in ultrathin cryosections, from tissue fixed only in paraformaldehyde, that provides excellent contrast at the electron microscopic level.