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Related Experiment Videos

Mitotic clonal expansion: a synchronous process required for adipogenesis.

Qi-Qun Tang1, Tamara C Otto, M Daniel Lane

  • 1Department of Biological Chemistry, Division of Endocrinology, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA. qtang1@jhem.jhmi.edu

Proceedings of the National Academy of Sciences of the United States of America
|December 28, 2002
PubMed
Summary

Mitotic clonal expansion (MCE) is essential for 3T3-L1 preadipocyte differentiation into adipocytes. Inhibiting cell cycle progression blocks this crucial expansion and subsequent adipogenesis.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • 3T3-L1 preadipocytes are a widely used model for studying adipogenesis.
  • Adipogenesis involves cell cycle re-entry, mitotic clonal expansion (MCE), and terminal differentiation.

Purpose of the Study:

  • To investigate the role of mitotic clonal expansion (MCE) in 3T3-L1 preadipocyte differentiation.
  • To determine the molecular mechanisms regulating MCE and adipogenesis.

Main Methods:

  • Induction of differentiation in growth-arrested 3T3-L1 preadipocytes.
  • Cell cycle analysis using markers like cdk2-cyclin-EA, p27kip1, Rb phosphorylation, and thymidine incorporation.
  • Pharmacological inhibition of MEK (PD98059, UO126) and cyclin-dependent kinases (roscovitine).

Related Experiment Videos

  • Assessment of gene expression for adipocyte markers and CEBPbeta DNA-binding activity.

    • Main Results:

    • 3T3-L1 preadipocytes synchronously reenter the cell cycle and undergo MCE upon differentiation induction.
    • CEBPbeta acquires DNA-binding activity as cells cross the G1/S checkpoint, initiating adipocyte gene expression.
    • MEK inhibition (PD98059) delayed MCE and differentiation, while more potent inhibitors (UO126, roscovitine) blocked MCE and adipogenesis.
    • MCE was confirmed as a prerequisite for adipocyte differentiation.

    Conclusions:

    • Mitotic clonal expansion (MCE) is a critical and necessary step for 3T3-L1 preadipocyte differentiation.
    • Cell cycle progression, regulated by kinases like MEK and CDKs, is essential for initiating adipogenesis.
    • Targeting cell cycle regulators offers potential strategies for controlling adipogenesis.