Related Experiment Videos
Simian virus 40 sequences in human lymphoblastoid B-cell lines
Riccardo Dolcetti1, Fernanda Martini, Michele Quaia
1Divisions of Experimental Oncology, Centro di Riferimento Oncologico, IRCCS, 33081 Aviano (Pordenone), Italy.
Journal of Virology
|December 28, 2002
Summary
Simian virus 40 (SV40) DNA was detected in over half of human lymphoblastoid B-cell lines. SV40-positive cell lines exhibited increased tumorigenicity, suggesting a potential role for SV40 in human lymphomagenesis.
Area of Science:
- Virology
- Oncology
- Immunology
Background:
- Epstein-Barr virus (EBV) immortalized lymphoblastoid B-cell lines are a model for B-cell studies.
- Simian virus 40 (SV40) has been investigated for its potential oncogenic properties in humans.
Purpose of the Study:
- To investigate the presence and potential role of SV40 in human lymphoblastoid B-cell lines.
- To assess the impact of SV40 infection on B-cell line tumorigenicity.
Main Methods:
- Polymerase Chain Reaction (PCR) for SV40 DNA detection.
- In situ hybridization to localize SV40 DNA within cells.
- Reverse transcription-PCR (RT-PCR) for SV40 T-antigen mRNA.
- Tumorigenicity assays in SCID mice.
Main Results:
- SV40 DNA was detected in 52.3% of human lymphoblastoid B-cell lines.
- SV40 DNA and T-antigen mRNA were found in B-cell nuclei.
- SV40-positive B-cell lines demonstrated higher tumorigenicity in SCID mice compared to SV40-negative lines.
Conclusions:
- SV40 DNA is present in a significant proportion of human lymphoblastoid B-cell lines.
- SV40 infection may enhance the tumorigenic potential of B-cells.
- These findings suggest SV40 could be implicated in the early stages of human lymphomagenesis.