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Yellow fever virus replicons as an expression system for hepatitis C virus structural proteins
Richard Molenkamp1, Engbert A Kooi, Marjoleine A Lucassen
1Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, The Netherlands.
Abstract:
Chimeric yellow fever virus (YF) RNAs were constructed in which the YF structural genes were replaced by the hepatitis C virus (HCV) structural genes or fusions between the YF and HCV structural genes. Interestingly, RNA replication required nucleotide complementarity between the 3'-located conserved sequence 1 and an RNA sequence located in the 5' end of the YF capsid sequence. The (chimeric-)HCV structural proteins were efficiently expressed and processed, and the native E1/E2 heterodimer was formed. However, no indication for the production of HCV-like particles was obtained.