Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Express primer tool for high-throughput gene cloning and expression.

J R Yoon1, P D Laible, M Gu

  • 1Argonne National Laboratory, Argonne, IL, USA.

Biotechniques
|December 31, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integration of transcriptomic data identifies CD163 as a key link between chronic rhinosinusitis with nasal polyps and COVID-19.

Rhinology·2026
Same author

[Clinical characteristics and prognostic factors of epidermal growth factor receptor-mutated non-small cell lung cancer transformed into small-cell lung cancer after treatment].

Zhonghua yi xue za zhi·2024
Same author

Significance of MRI-based radiomics in predicting pathological complete response to neoadjuvant chemoradiotherapy of locally advanced rectal cancer: A narrative review.

Cancer radiotherapie : journal de la Societe francaise de radiotherapie oncologique·2024
Same author

[The Textual Relationship between <i>Zhongguo Yixue Dacidian</i> and <i>YiJi ZhiJin</i>].

Zhonghua yi shi za zhi (Beijing, China : 1980)·2024
Same author

[The application of full-length urethral preservation without anastomosis in single-port laparoscopic radical prostate cancer].

Zhonghua wai ke za zhi [Chinese journal of surgery]·2024
Same author

Prognostic Value of Four Preimplantation Malnutrition Estimation Tools in Predicting Heart Failure Hospitalization of the Older Diabetic Patients with Right Ventricular Pacing.

The journal of nutrition, health & aging·2023

A new web tool designs gene cloning primers for efficient expression clone generation. This simplifies creating multiple primers for simultaneous gene amplification in high-throughput projects.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Synthetic Biology

Background:

  • High-throughput gene cloning and expression necessitate advanced molecular tools.
  • Current methods may require significant manual intervention for primer design.

Purpose of the Study:

  • To introduce a web-based tool for designing primers for expression clone generation.
  • To support both laboratory-scale and high-throughput molecular biology projects.
  • To minimize manual effort in designing primers for multiple gene amplifications.

Main Methods:

  • Development of a flexible, web-based primer design application.
  • Incorporation of user-defined primer design parameters.
  • Facilitation of simultaneous amplification of multiple target genes.

Related Experiment Videos

Main Results:

  • A novel tool enabling customized primer design for expression cloning.
  • Reduced manual workload for generating numerous primers.
  • Efficient amplification of multiple genes for cloning and expression.

Conclusions:

  • The developed tool enhances efficiency in gene cloning and expression workflows.
  • It provides a flexible and user-friendly solution for primer design in molecular biology.
  • The application supports the advancement of high-throughput biological research.