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Leaky Scanning

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During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...

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Related Experiment Video

Updated: Jul 21, 2026

Rescue of Recombinant Newcastle Disease Virus from cDNA

Rescue of Recombinant Newcastle Disease Virus from cDNA

Published on: October 11, 2013

Semliki forest virus-based expression for versatile use in receptor research.

Kenneth Lundstrom¹

¹Regulon Inc., Biopole Epalinges, Les Croisettes 22, CH-1066 Epalinges/Lausanne, Switzerland. kenneth@regulon.org

Journal of Receptor and Signal Transduction Research

|December 31, 2002

Summary

Semliki Forest virus (SFV) vectors enable efficient gene expression in mammalian cells and large-scale protein production. Engineered SFV vectors are valuable tools for neurobiology and drug discovery.

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Area of Science:

Molecular Biology
Virology
Neuroscience

Background:

Semliki Forest virus (SFV) vectors are established tools for gene expression studies.
SFV vectors offer high efficiency in various mammalian cell types.

Purpose of the Study:

To highlight the versatility and applications of SFV vectors in biological research.
To showcase advancements in SFV vector technology for enhanced utility.

Main Methods:

Development of SFV vectors for gene expression in mammalian cells.
Scaling up SFV expression systems for protein production.
Engineering of novel SFV mutant vectors with improved properties.

Main Results:

SFV vectors demonstrate high efficiency in both immortalized and primary mammalian cells.
Scalable SFV expression facilitates large-scale recombinant protein production for drug screening.
SFV vectors exhibit preferential expression in neuronal cells, benefiting neurobiological research.
Novel SFV mutants show reduced cytotoxicity and temperature sensitivity, expanding applications.

Conclusions:

SFV vectors are powerful and adaptable tools for gene expression and protein production.
SFV vector technology advancements continue to broaden their utility in diverse research areas, including neuroscience and drug discovery.