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Cyclooxygenase (COX)-2 and granulosa cell apoptosis in vitro
Angela S Caffrey1, William C Patton, Johannah U Corselli
1Department of Gynecology and Obstetrics, Loma Linda University School of Medicine, Loma Linda, California 92350, USA.
Purpose:
C-myc was studied in cyclooxygenase (COX)-2 associated granulosa cell apoptosis,
Methods:
Granulosa cells (N = 5 cases) were incubated for 24 h in either 1 or 50 microM COX-2 inhibitor, 1 or 50 microM COX-1/COX-2 inhibitor, negative or positive controls Single primer polymerase chain reaction of c-myc exon 1 were performed. Bisbenzimide-stained control single-stranded (ssDNA) were hybridized to SYBR Gold-stained ssDNA and fluorescent images analyzed.
Results:
C-myc was disrupted by the high-dose COX-2 inhibitor. Cell viability decreased with COX-1 and COX-2 inhibition. However, cell viability was similar for the positive control and at low-dose COX-2 inhibition.
Conclusions:
Inhibition of both COX-1 and COX-2 initiated apoptosis without disrupting c-myc suggesting a protective effect on c-myc. The low dosage of the COX-2 inhibitor did not disrupt c-myc and cell viability. C-myc sensitization was not part of apoptosis.