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Reiteration frequency of the gene for tissue-specific histone H5 in the chicken genome

A C Scott, J R Wells

    Nature
    |February 26, 1976
    PubMed

    Insights

    Researchers isolated the mRNA for chicken-specific histone H5 from reticulocytes. This complementary DNA (cDNA) analysis revealed that the H5 histone gene is repeated 10 times in the chicken genome.

    Area of Science:

    • Molecular Biology
    • Genomics
    • Cell Biology

    Background:

    • Erythroid cells possess unique histone proteins, such as chicken H5, in addition to the standard five histone types.
    • Histone H5 is a tissue-specific protein found in chicken red blood cells.

    Purpose of the Study:

    • To isolate and characterize the messenger RNA (mRNA) encoding the chicken-specific histone H5.
    • To determine the gene copy number for histone H5 in the chicken genome.

    Main Methods:

    • Indirect immunoprecipitation was used to isolate histone H5 mRNA from immature reticulocytes.
    • Enzymatic addition of a polyadenylic acid tract to the mRNA.
    • Complementary DNA (cDNA) synthesis using avian myeloblastosis virus reverse transcriptase.
    • Hybridization studies to analyze cDNA.

    Main Results:

    • Histone H5 mRNA was successfully isolated from chicken reticulocytes.
    • The gene coding for histone H5 is reiterated approximately 10 times within the chicken genome.

    Conclusions:

    • The study successfully isolated and transcribed the mRNA for chicken histone H5.
    • The H5 histone gene is present in multiple copies in the chicken genome, suggesting potential regulatory mechanisms or gene families.

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