Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A cross-platform public domain PC image-analysis program for the comet assay.

Krzysztof Końca1, Anna Lankoff, Anna Banasik

  • 1Institute of Theoretical Physics, University of Wrocław, Poland.

Mutation Research
|December 31, 2002
PubMed
Summary

A new, free software program called CASP analyzes DNA damage using the comet assay. This tool, designed for PCs, offers reproducible results for genotoxicity and DNA repair studies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inhibiting biofilm growth on ammonium salt-functionalized or fluorinated voice prostheses silicone.

Applied microbiology and biotechnology·2026
Same author

The Declaration of Adelaide White paper: Guidelines for Author Responsibility in Peer Review.

Journal of radiological protection : official journal of the Society for Radiological Protection·2026
Same author

Assessing the variation of DNA damage response of individual human cells exposed to photon radiation.

Radiation and environmental biophysics·2026
Same author

Assessment of biofilm growth on voice prostheses using a tracheoesophageal fistula simulator.

Otolaryngologia polska = The Polish otolaryngology·2026
Same author

Chromosomal damage in breast cancer patients undergoing different radiotherapy schemes.

International journal of radiation biology·2026
Same author

Effectiveness of pelvic floor rehabilitation after radical prostatectomy and continence recovery in relation to surgical technique.

Scientific reports·2026

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • The comet assay is a popular method for assessing genotoxicity, cytotoxicity, and DNA repair kinetics.
  • Analysis of comet assay images is typically done using computer programs, but commercial options are expensive and lack source code access.
  • Existing free options are limited, particularly for the PC platform.

Purpose of the Study:

  • To develop a cost-free, public domain software program for analyzing comet assay data on PC computers.
  • To provide an accessible and reliable tool for genotoxicity and DNA repair research.

Main Methods:

  • Development of a public domain software program named CASP under the GNU license for PC computers.
  • Testing the software's efficacy using human lymphocytes exposed to gamma-rays.

Related Experiment Videos

  • The program is designed to analyze images of damaged cells, forming a comet-like structure.

    • Main Results:

    • CASP provides reproducible results when analyzing DNA damage in human lymphocytes.
    • The software is compatible with various hardware and software platforms, including Windows 95 and Linux.
    • The program facilitates the analysis of genotoxic and cytotoxic effects and DNA repair kinetics.

    Conclusions:

    • CASP offers a cost-free, accessible, and reliable solution for comet assay analysis on the PC platform.
    • The developed software aids in the study of DNA damage and repair mechanisms.
    • Availability of source code and binaries promotes wider adoption and research collaboration.