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Related Experiment Videos

Invariant Sema5A inhibition serves an ensheathing function during optic nerve development.

Stephen F Oster1, MacDara O Bodeker, Fengling He

  • 1Department of Ophthalmology, Medical Scientist Training Program, University of California San Francisco, CA 94143, USA.

Development (Cambridge, England)
|December 31, 2002
PubMed
Summary

Semaphorin5A (Sema5A) acts as an inhibitory guidance cue, preventing retinal axons from straying during optic nerve development. This finding highlights the role of inhibitory molecules in maintaining the integrity of central nervous system (CNS) nerve pathways.

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Molecular Biology

Background:

  • Axon pathfinding is crucial for neural circuit formation.
  • Known guidance molecules like L1, laminin, and netrin-1 regulate retinal axon navigation.
  • The role of additional guidance cues in the optic nerve remains to be fully elucidated.

Purpose of the Study:

  • To identify novel molecules involved in embryonic mouse retinal axon pathfinding.
  • To investigate the function and regulation of Semaphorin5A (Sema5A) in retinal axon guidance.
  • To determine how Sema5A interacts with other guidance cues.

Main Methods:

  • Analysis of Semaphorin5A (Sema5A) mRNA and protein expression in embryonic mouse optic disc and nerve.
  • In vitro assays including growth cone collapse, substratum choice, and neurite outgrowth.

Related Experiment Videos

  • In vivo antibody-perturbation studies in living embryo preparations.

    • Main Results:

    • Semaphorin5A (Sema5A) is expressed in neuroepithelial cells surrounding retinal axons at the optic disc and optic nerve.
    • Sema5A consistently inhibited retinal axon growth in the presence of L1, laminin, or netrin-1.
    • Blocking Sema5A function caused retinal axons to deviate from the optic nerve bundle.

    Conclusions:

    • Semaphorin5A (Sema5A) acts as an inhibitory guidance cue essential for maintaining the integrity of the retinal pathway within the optic nerve.
    • Inhibitory molecular sheaths are critical for the proper development of some central nervous system (CNS) nerves.
    • Sema5A demonstrates conserved inhibitory responses when co-exposed to multiple guidance molecules, suggesting a general mechanism for axon pathfinding regulation.