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Related Experiment Videos

Experimental keratomycosis in a mouse model.

Tzu G Wu1, Kirk R Wilhelmus, Bradley M Mitchell

  • 1Sid W. Richardson Ocular Microbiology Laboratory, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, 6565 Fannin, Houston, TX 77030, USA.

Investigative Ophthalmology & Visual Science
|December 31, 2002
PubMed
Summary

This study establishes a new mouse model for studying fungal keratitis caused by Candida albicans. Immunocompromised mice developed severe keratomycosis, aiding research into fungal eye infections.

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Area of Science:

  • Ophthalmology
  • Mycology
  • Immunology

Background:

  • Ocular fungal infections, particularly keratitis caused by Candida albicans, pose a significant threat to vision.
  • Understanding the pathogenesis of fungal keratitis requires robust experimental models for studying fungal adherence and invasion.

Purpose of the Study:

  • To establish a murine model of corneal candidiasis for molecular evaluation of fungal adherence and invasion.
  • To investigate the role of immunosuppression in the severity and persistence of Candida albicans corneal infections.

Main Methods:

  • Corneal scarification followed by topical inoculation with varying doses of Candida albicans in immunocompetent and immunosuppressed mice (methylprednisolone or cyclophosphamide).
  • Disease severity was assessed daily, and eyes were analyzed via quantitative fungal cultures, PCR for candidal DNA, and histopathology.

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Main Results:

  • A minimum of 1 million colony-forming units (CFU) of C. albicans was required to consistently induce keratitis.
  • Immunosuppression with methylprednisolone or cyclophosphamide significantly increased disease severity and fungal persistence in both mouse strains.
  • Histopathology confirmed the presence of C. albicans, inflammation, and stromal necrosis in infected corneas.

Conclusions:

  • A murine model of moderate to severe keratomycosis can be successfully established in immunocompromised mice via corneal scarification.
  • This model provides a foundation for further research into the pathogenesis of fungal eye infections and the efficacy of antifungal strategies.