Related Experiment Video
Updated: Sep 27, 2026

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization
Published on: October 29, 2019
The two-dimensional and three-dimensional processing of images provided by conventional microscopes
1Department of Mathematics, Faculty of Mechanical Engineering, Brno University of Technology, Brno, Czech Republic. martisek@mat.fme.vutbr.cz
Abstract:
In this paper, I present an interesting processing method of microscopic images. High-pass type filters are generally used for image focusing. They enhance the high spatial frequencies. They are, however, efficient only in cases when the picture is not sharp because of the low contrast on high frequencies (for example in a TV picture). These filters, are not appropriate if the lack of sharpness has been caused by other factors. In this case, it is not possible to construct a three-dimensional model of the observed object. Better results and a three-dimensional model can be obtained by applying the following theory. As part of this paper, an original program based on this theory is described.
Related Concept Videos
Two-Dimensional Microscopy in Microbiology
Three-Dimensional Microscopy in Microbiology
Overview of Microscopy Techniques
Overview of Electron Microscopy
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

