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Angiogenesis assays: a critical overview.

Robert Auerbach¹, Rachel Lewis, Brenda Shinners

¹Laboratory of Developmental Biology, University of Wisconsin, 1117 West Johnson St., Madison, WI 53706, USA. rauerbach@facstff.wisc.edu

Clinical Chemistry

|January 1, 2003

Summary

Angiogenesis assays evaluate agents affecting new blood vessel formation. While in vitro methods offer speed and quantification, in vivo assays are crucial for confirming complex vascular responses.

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Area of Science:

Biomedical research
Vascular biology

Background:

Angiogenesis, or new blood vessel formation, is vital in development and disease, including cancer and ocular conditions.
Angiogenesis assays are critical for assessing pro- and antiangiogenic agents.

Purpose of the Study:

To describe principal in vitro and in vivo methods for studying angiogenesis.
To introduce novel chick aortic arch and Matrigel sponge assays.

Main Methods:

In vivo assays: Matrigel plug, corneal neovascularization, chick chorioallantoic membrane (CAM).
In vitro assays: cellular proliferation, migration, tube formation, and organotypic aortic ring.
New methods: chick aortic arch and Matrigel sponge assays.

Main Results:

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In vitro assays provide rapid, quantifiable initial data.
In vivo assays are essential for validating complex vascular responses.
Multiple assay types are recommended for comprehensive evaluation.

Conclusions:

In vitro angiogenesis tests are valuable but require cautious interpretation.
In vivo assays are indispensable for confirming findings due to their ability to capture complex vascular dynamics.
Combining in vitro and in vivo methods maximizes the benefit of angiogenesis studies.