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Plasma membranes from experimental granulation tissue.

P Lehtinen, E Vuorio, E Kulonen

    The Biochemical Journal
    |March 1, 1975
    PubMed
    Summary
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    Researchers developed a new method to isolate rat plasma membranes without enzymes, achieving high yield and purity. These membranes show distinct protein and lipid profiles during tissue development and differ from other cell types.

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Membrane Biology

    Background:

    • Plasma membranes are crucial for cellular function and signaling.
    • Understanding plasma membrane composition changes during tissue development is important.

    Purpose of the Study:

    • To develop an enzyme-free procedure for isolating rat plasma membranes from granulation tissue.
    • To characterize the biochemical and compositional properties of these membranes.
    • To compare plasma membranes from granulation tissue with those from other cell types.

    Main Methods:

    • Enzyme-free isolation of plasma membranes from rat granulation tissue.
    • Biochemical assays for enzyme activities (5'-nucleotidase, Na+, K+-activated Mg2+-dependent adenosine triphosphatase, leucine beta-naphthylamidase).

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  • Analysis of lipid composition and gel electrophoresis for protein and glycoprotein profiling.
  • Comparison with plasma membranes from rat peritoneal macrophages and embryonic-chick tendon cells.

    • Main Results:

    • Achieved >20% yield and tenfold purification of plasma membranes without enzymes.
    • Identified differences in protein/lipid ratios and specific enzyme activities between mature and proliferating granulation tissue membranes.
    • Observed distinct gel-electrophoretic patterns corresponding to different developmental phases.

    Conclusions:

    • The developed method provides a reliable way to obtain purified plasma membranes.
    • Plasma membrane composition and enzyme activity vary with granulation tissue development.
    • Granulation tissue plasma membranes exhibit unique characteristics compared to macrophages and tendon cells.