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PCR analysis of Yq microdeletions in infertile males, a study from South India
S Ramesh Babu1, M Swarna, P Padmavathi
1Institute of Genetics & Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad-500 016, India.
Aim:
To estimate the frequency of microdeletions in the long arm of Y-chromosome of 20 infertile males from South India.
Methods:
Polymerase chain reaction (PCR) amplification using Y-specific STS of azoospermia factor (AZF) regions i.e., SY 84 for AZFa, SY 127 for AZFb and SY 254 for AZFc.
Results:
Of the 20 infertile subjects 3 (15 %), one azoospermic and two oligozoospermic, showed microdeletions in the AZF region of Y-chromosome.
Conclusion:
The frequency of deletions involving AZF region of the Y-chromosome is 15 % in azoospermic and severely oligozoospermic infertile men. PCR amplification of AZF locus is useful for the diagnosis of microdeletions in the Y-chromosome.
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