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Role of NF-kB in multiple organ dysfunction during acute obstructive cholangitis
Bin Tu1, Jian-Ping Gong, Hu-Yi Feng
1Department of General Surgery, the Second College of Clinical Medicine and the Second Affiliated Hospital of Chongqing University of Medical Science, 74 Linjiang Road, Chongqing 400010, China.
World Journal of Gastroenterology
|January 1, 2003
Summary
NF-kB activation is implicated in multiple organ dysfunction (MOD) during acute obstructive cholangitis (AOC). Blocking bile duct ligation significantly reduced organ damage and NF-kB activation in rats, highlighting a key therapeutic target.
Area of Science:
- Gastroenterology and Hepatology
- Immunology
- Pathology
Background:
- Acute obstructive cholangitis (AOC) can lead to multiple organ dysfunction (MOD).
- The role of nuclear factor kappa B (NF-kB) activation in MOD during AOC requires further elucidation.
Purpose of the Study:
- To investigate the involvement of NF-kB activation in the development of MOD during AOC in a rat model.
- To assess the impact of bile duct ligation (BDL) on organ damage and NF-kB activation.
Main Methods:
- Forty-two Wistar rats were allocated to AOC, BDL, or sham operation (SO) groups.
- Organ morphology was examined using light and electron microscopy.
- NF-kB activation was quantified via Electrophoretic Mobility Shift Assay (EMSA).
- Arterial blood gas analysis, serum markers (LDH, ALT, BUN, creatinine), and plasma cytokines (TNF-alpha, IL-6) were measured.
Main Results:
- AOC induced significant histological and ultrastructural damage in vital organs.
- NF-kB activation was markedly increased in the AOC group compared to BDL and SO groups.
- AOC rats exhibited elevated levels of LDH, ALT, BUN, creatinine, TNF-alpha, and IL-6.
- BDL intervention significantly attenuated organ damage and reduced inflammatory markers.
Conclusions:
- NF-kB activation plays a critical role in the pathological damage observed in vital organs during AOC.
- These findings suggest that targeting NF-kB activation could be a potential therapeutic strategy for managing MOD in AOC.