[Overexpression of truncated type II transforming growth factor-beta receptor in dermal fibroblasts]
Wei Liu1, Ze-hao Cai, Qing-xin Shang
1Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Second Medical University, Shanghai, P. R. China 200011. liuwei-2000@yahoo.com
Objective:
To study the effect of overexpression of truncated type II TGF-beta receptor on transforming growth factor-beta 1(TGF-beta 1) autoproduction in normal dermal fibroblasts.
Methods:
In vitro cultured dermal fibroblasts were treated with recombinant human TGF-beta 1(rhTGF-beta 1) (5 ng/ml) or recombinant adenovirus containing truncated type II TGF-beta receptor gene (50 pfu/cell). Their effects on regulating gene expression of TGF-beta 1 were observed with Northern blotting.
Results:
rhTGF-beta 1 up-regulated the gene expression of TGF-beta 1 and type I procollagen. Overexpression of truncated receptor II down-regulated the gene expression of TGF-beta 1.
Conclusion:
Overexpression of the truncated TGF-beta receptor II decreases TGF-beta 1 autoproduction via blocking TGF-beta receptor signal. The results may provided a new strategy for scar gene therapy.
Related Concept Videos
Factors Influencing Microbial Growth: pH
Types Of Transformers
If the ratio of the number of turns in the secondary winding to that of the primary winding is greater than one, then the transformer is said to be a step-up transformer. In a step-up transformer, the voltage at the secondary winding is greater than the voltage applied at the primary winding.
However, if this ratio is less than one, the transformer is said to be a step-down...
Types of Receptors: Internal Receptors
Similar to membrane-bound receptors, the binding of a ligand to the intracellular receptor of causes a conformational change in the...
Types of Receptors: Cell Surface Receptors
Truncation in Survival Analysis
Left truncation occurs when individuals who experienced the event of interest before a certain time are not included in the study. This is often due to a "delayed entry" into the study where only those who survive until a certain entry point are...
Role of Hematopoietic Growth Factors
Thrombopoietin (TPO), mainly released by the liver,...


