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Genetically engineered stem rust resistance in barley using the Rpg1 gene.

Henriette Horvath¹, Nils Rostoks, Robert Brueggeman

¹Department of Crop and Soil Sciences, Washington State University, Pullman 99164, USA.

Proceedings of the National Academy of Sciences of the United States of America

|January 2, 2003

Summary

Researchers transformed susceptible barley with the Rpg1 gene, successfully conferring stem rust resistance. This study confirms Rpg1

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Area of Science:

Plant genetics
Crop science
Molecular biology

Background:

Stem rust, caused by Puccinia graminis f. sp. tritici, poses a significant threat to barley cultivation.
The Rpg1 gene is known to confer resistance to stem rust in barley.

Purpose of the Study:

To confirm the functionality of the cloned Rpg1 gene in conferring stem rust resistance.
To assess the efficacy of Rpg1 gene transformation in a susceptible barley cultivar.

Main Methods:

Agrobacterium-mediated transformation of barley cv. Golden Promise using the Rpg1 genomic clone.
Seedling infection assays with stem rust pathotype Pgt-MCC.
Southern blot and RT-PCR analysis to examine gene integration and expression.

Main Results:

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Transformed Golden Promise barley exhibited high resistance to stem rust, converting it from a susceptible to a resistant cultivar.
A single copy of the Rpg1 gene was sufficient for resistance, with segregation ratios indicating Mendelian inheritance.
Transgenic lines showed enhanced resistance compared to original Rpg1 sources, with no visible infection or pathogen sporulation.

Conclusions:

The cloned DNA segment is the functional Rpg1 gene responsible for stem rust resistance in barley.
Transformation with the Rpg1 gene is an effective strategy to develop stem rust-resistant barley varieties.
The enhanced resistance in transformants warrants further investigation into underlying mechanisms beyond gene copy number or expression.