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Freezing Human ES Cells
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Freezing Human ES Cells

Published on: October 12, 2006

Cell biology in the Antarctic: studying life in the freezer

Karin Römisch1, Tom Matheson

  • 1Cambridge Institute of Medical Research and the Department of Clinical Biochemistry University of Cambridge, Hills Road, Cambridge, CB2 2XY, UK. kbr20@cam.ac.uk

Nature Cell Biology
|January 4, 2003
PubMed

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...