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[A study on quality standard for herba cistanches].

S J Zhang1, L Liu, S Y Zhang

  • 1Institute of Chinese Materia Madica, China Academy of TCM, Beijing 100070, China.

Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica
|January 7, 2003
PubMed
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This study establishes a quality standard for Herba Cistanches using thin-layer chromatography (TLC) and RP-HPLC. The developed method accurately identifies and quantifies key active components for reliable quality control of cistanche deserticola.

Area of Science:

  • Pharmacognosy
  • Analytical Chemistry
  • Traditional Chinese Medicine

Background:

  • Herba Cistanches is a crucial traditional Chinese medicine.
  • Establishing a robust quality standard is essential for its therapeutic efficacy and safety.
  • Variability in Herba Cistanches necessitates reliable quality control methods.

Purpose of the Study:

  • To develop and validate a comprehensive quality standard for Herba Cistanches.
  • To ensure the consistent quality and authenticity of Herba Cistanches used in medicinal applications.
  • To provide a reliable method for the quality control of cistanche deserticola.

Main Methods:

  • Qualitative identification of five active components (acteoside, echinacoside, cistanoside A, betaine, and mannitol) using Thin-Layer Chromatography (TLC).

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  • Quantitative analysis of acteoside content utilizing Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC).
  • Application of the developed methods to fourteen types of Herba Cistanches and eleven types of Yinpian.

    • Main Results:

    • A validated TLC method for qualitative identification of key active components was successfully established.
    • RP-HPLC enabled accurate quantification of acteoside content in Herba Cistanches samples.
    • The developed analytical methods demonstrated high accuracy, reliability, good separability, and reproducibility.

    Conclusions:

    • The established method serves as a reliable standard for the quality control of Herba Cistanches.
    • This quality standard is crucial for ensuring the safety and efficacy of cistanche deserticola in pharmaceutical preparations.
    • The validated analytical techniques provide a foundation for consistent Herba Cistanches quality in the market.