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Published on: March 27, 2014
[Studies on separation and identification of Rhizoma coptidis brugs based on microemulsion thin-layer chromatography]
1Yangzhou Municipal Institute for Drug Control, Yangzhou 225009, Jiangsu, China.
Objective:
To investigate the use of microemulsion TLC for the separation and identification of Rhizoma Coptidis drugs.
Method:
Thirteen Rhizoma Coptidis drugs were separated and identified with six kinds of SDS/n-C4H9OH/n-C7H16/H2O microemulsions as mobile phase on polyamide film. Effects of microemulsions on separation were investigated.
Results:
The best mobile phase was the O/W microemulsion(containing 75% water). Compared with general mobile phases, the separation and detection could be improved and the test conditions were more tolerant.
Conclusion:
This new method is simple, accurate and efficient for the separation and identification of Rhizoma Coptidis drugs.
