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Related Experiment Videos

Cholesterol removal from media by lactococci.

H Kimoto1, S Ohmomo, T Okamoto

  • 1Department of Animal Products, National Institute of Livestock and Grassland Science, Tsukuba Norin-danchi, P.O. Box 5, Ibaraki 305-0901, Japan. anne@niai.affrc.go.jp

Journal of Dairy Science
|January 7, 2003
PubMed
Summary

Certain lactococci strains remove cholesterol from growth media, with Lc. Lactis N7 showing significant potential for reducing serum cholesterol levels in vivo.

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Area of Science:

  • Microbiology
  • Biochemistry
  • Probiotics

Background:

  • Elevated serum cholesterol is a risk factor for coronary heart disease.
  • Lactobacillus acidophilus has demonstrated hypocholesterolemic effects by removing cholesterol.
  • Investigating other bacterial genera for similar cholesterol-lowering properties is warranted.

Purpose of the Study:

  • To evaluate cholesterol removal capabilities of Lactococcus strains.
  • To identify specific Lactococcus strains with significant cholesterol-binding activity.
  • To assess the potential of Lactococcus strains as probiotics for managing cholesterol levels.

Main Methods:

  • Culturing seven Lactococcus strains in cholesterol-containing media.
  • Quantifying cholesterol removal without degradation.

Related Experiment Videos

  • Analyzing growth characteristics and cell composition of cholesterol-removing strains.
  • Comparing cholesterol removal by viable and heat-killed cells.

    • Main Results:

    • All tested Lactococcus strains removed cholesterol from media without degradation.
    • Cholesterol removal varied among strains; Lc. Lactis subsp. lactis biovar diacetylactis N7 showed high efficacy.
    • Strain N7 exhibited enhanced growth and altered fatty acid composition when removing cholesterol.
    • Cholesterol removal was observed in both viable and heat-killed N7 cells, but higher in viable cells.

    Conclusions:

    • Lc. Lactis N7 demonstrates significant cholesterol removal capacity, comparable to known beneficial strains.
    • The ability of strain N7 to remove cholesterol is independent of cell viability.
    • Strain N7 shows promise as a probiotic for in vivo cholesterol management and hypocholesterolemic effects.