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Updated: Aug 1, 2026

An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection
Published on: December 10, 2013
[Human herpesvirus-6 infection induces IL-10 production in monocytes]
1Children's Hospital, Chongqing University of Medical Sciences, Chongqing 630014.
Abstract:
In this report, interleukin 10(IL-10) and other cytokines were evaluated through reverse transcription-polymerase chain reaction (RT-PCR) and sandwich ELISA to explore possible immune pathogenesis of human herpesvirus 6 (HHV-6) infection. The results showed that IL-10 was expressed in HHV-6 infected monocytes. Kinetic studies found that transcription of IL-1 beta, IL-6 and TNF-alpha mRNA decreased with accumulation of IL-10 mRNA. When endogenous IL-10 induced by HHV-6 was blocked using anti-human IL-10 monoclonal antibody, the production and the expression of TNF-alpha, IL-1 beta and IL-6 markedly increased as compared with infection by HHV-6 alone, indicating that endogenous IL-10 inhibited cytokine production at the transcription level in HHV-6 infected monocytes. IL-10 has been shown to suppress Th1 response and down-regulate monocyte/macrophage function. The data from our studies suggest that disturbance of immune function and latent infection resulting from HHV-6 infection might partially contribute to endogenous IL-10 production.
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