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[Extraction and isolation of active component for inhibiting PC3 cell proliferation in vitro from the fruit of Lycium
1Institute of Materia Medica, Zhejiang Academy of Medical Sciences1, Hangzhou 310013, Zhejiang, China.
Objective:
To extract and isolate the active component inhibiting PC3 cell proliferation in vitro from the fruit of Lycium barbarum.
Method:
The active components of the fruit of L. barbarum were extracted by water, 95% ethanol, 50% ethanol respectively. Then, ethanol extract was extracted with acetone and n-butanol and isolated by SiO2 column and purified by TLC to obtain the active component. The chemical structure of active component was identified by the spectral analysis. MTT assay was used to compare the inhibition activity(IC50) of PC3 cells proliferation of the all extracts.
Result:
According IC50, the most active component among all extracts isolated from the fruit of L. barbarum isscopoletin.
Conclusion:
Scopoletin is the active component of the fruit of L. barbarum for inhibiting PC3 cell proliferation.
