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[Studies on method for the determination of kaerophyllin in Bupleurum smithii wolff Wolff var. parvifolium]
1School of Pharmaceutical Sciencn, West China University of Medical Sciences, Chengdu 610041, Sichuan, China.
Objective:
To establish a quantitative method for the determination of kaerophyllin in Chinese medicine Bupleurum smithii ver. parvifolium.
Method:
Kaerophyllin was determined by HPLC, the mobile phase consisted of methanol-water(70:30), the flow rate was 1 ml.min-1, the eluate was monitored with UV detector at 328 nm.
Result:
The average recovery was 97.1% with RSD = 2.15 (n = 5).
Conclusion:
The result was precise and stable. This method was sensitive and its repeatability was excellent. This method may serve determination of kaerophyllin in B. smithii var. parvifolium.
