Comparison of different retention models in normal- and reversed-phase liquid chromatography with binary mobile phases
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Updated: Sep 27, 2026

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Published on: March 8, 2020
Wojciech Zapala1, Krzysztof Kaczmarski, Teresa Kowalska
1Department of Chemical Engineering and Process Control, Chemical Faculty, Rzeszów University of Technology, ul. Powstańców Warszawy 6, 35-959 Rzeszów, Poland. ichwz@prz.rzeszow.pl
Abstract:
The dependence on mobile phase composition of the retention of selected test analytes in different normal- and reversed-phase chromatographic systems is studied. The aim of this study is to compare the performance of six valuable retention models reported in the literature with a new empirical equation, which is first introduced in this study. All of these models are compared for different thin-layer chromatographic and high-performance liquid chromatographic systems by use of three criteria: the sum of the squared differences between the experimental and theoretical data, approximation of the standard deviation, and the Fisher test.
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