OPLC and HPTLC methods in physicochemical studies of a new group of antimycotic compounds

In this study two high-performance methods [overpressured layer chromatography (OPLC) and high-performance thin-layer chromatograpy (HPTLC) under reversed-phase conditions] are used to evaluate the hydrophobicity properties of newly synthesized bioactive compounds. The retention behavior of 24 2-(2,4-dihydroxyphenyl)benzothiazoles are examined for acquisition of log k data. With water-methanol mixtures as the mobile phases, the concentration range in which the correlation between log k and methanol content is linear is established for both methods and used to determine the hydrophobicity parameters of log kw by linear extrapolation. The effect of substituents on retention constants is quantitated by using the group contribution parameters (tauw). The results suggest the use of OPLC methods for quick analysis of physicochemical properties of a large number of organic compounds.