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Uncertainty in quantitative thin-layer chromatography.

Mirko Prosek1, Alenka Golc-Wondra, Irena Vovk

  • 1National Institute of Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia. mirko.prosek@ki.si

Journal of Chromatographic Science
|January 8, 2003
PubMed
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The error budget model, a metrological approach, can aid in planning thin-layer chromatographic (TLC) method validation. However, it cannot replace the full validation process for quantitative TLC analysis.

Area of Science:

  • Analytical Chemistry
  • Metrology

Background:

  • Globalization necessitates enhanced control over analytical measurement variability.
  • The error budget model, established by the Bureau International des Poids et Mesures, is a standard for measurement uncertainty but not directly applicable to separation techniques.
  • Quantitative measurement uncertainty in separation techniques remains a challenge.

Purpose of the Study:

  • To investigate the applicability of the error budget model as a planning tool for thin-layer chromatographic (TLC) method validation.
  • To develop and test a computer program for quantifying uncertainty components in quantitative TLC.
  • To assess whether the error budget approach can substitute traditional validation procedures.

Main Methods:

  • Experimental validation of the error budget model using thin-layer chromatography (TLC).

Related Experiment Videos

  • Development of a computer program to quantify uncertainty in quantitative TLC.
  • Testing with TLC and high-performance TLC plates, various samples, manual and automated application, and multiple scanning modes (UV-vis, video documentation, remission, transmission, fluorescence).

    • Main Results:

    • The error budget model can be utilized as a planning tool in the validation of quantitative TLC methods.
    • A computer program successfully quantitated uncertainty components in quantitative TLC.
    • Calculated uncertainty values using the error budget model were comparable to those from traditional validation procedures.

    Conclusions:

    • The error budget approach is a valuable planning tool for quantitative TLC method validation but cannot substitute the complete validation process.
    • While it can predict critical points in quantitative TLC, it does not confirm the validity of a chosen chromatographic procedure.