Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cell death induced by granzyme C.

Hillary Johnson1, Luca Scorrano, Stanley J Korsmeyer

  • 1Division of Oncology, Department of Medicine, Siteman Cancer Center, Washington University School of Medicine, St Louis, MO 63110, USA.

Blood
|January 8, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MitoCatch directs mitochondria delivery and prevents cell degeneration.

Cell research·2026
Same author

Long-read cDNA sequencing reveals novel isoforms and spliceosome-mutant-enriched transcripts in AML and MDS.

bioRxiv : the preprint server for biology·2026
Same author

Mitochondrial calcium uptake drives organelle remodeling to promote inflammasome-dependent cytokine release.

Cell death and differentiation·2026
Same author

FAS-controlled T cells drive lymphoproliferation through glycolysis without effector differentiation.

Journal of human immunity·2026
Same author

Corrigendum to "Recurrent Transcriptional Responses in AML and MDS patients Treated with Decitabine" Experimental Hematology. 2022;111:50-65.

Experimental hematology·2026
Same author

MFF budding from mitochondria regulates melanosome size and maturation.

Nature communications·2026
Same journal

Decentralized Clinical Trials in Hematology: the Promise and the Peril.

Blood·2026
Same journal

How I Treat Chemotherapy-Induced Thrombocytopenia with Thrombopoietin Receptor Agonists.

Blood·2026
Same journal

The Chaos of Choice in Large B-cell Lymphoma: A Call to Harmonize First-line Trial Design.

Blood·2026
Same journal

Precision Transfusion Medicine in the Omics Era.

Blood·2026
Same journal

Fibrocytes drive JAK2V617F-mutated myelofibrosis: pitavastatin reverses marrow fibrosis and anemia.

Blood·2026
Same journal

Identifying steroid-refractory aGVHD before it happens.

Blood·2026
See all related articles

Murine granzyme C (GzmC) induces rapid cell death through protease activity, affecting nuclear and mitochondrial targets. This mechanism differs from granzyme B

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • The functions of granzymes A and B in cytotoxic lymphocytes are well-defined.
  • The roles of other highly expressed murine granzymes (C, D, and F) remain largely uncharacterized.

Purpose of the Study:

  • To investigate the cell death-inducing capabilities of murine granzyme C (GzmC).
  • To elucidate the molecular mechanisms and cellular targets employed by GzmC.

Main Methods:

  • Assessed GzmC's ability to induce cell death in target cells.
  • Analyzed phosphatidylserine externalization, nuclear morphology, and DNA integrity.
  • Investigated caspase activation, BID cleavage, and CAD nuclease activity.
  • Examined mitochondrial integrity and membrane potential.

Related Experiment Videos

Main Results:

  • GzmC induces rapid cell death requiring protease activity, characterized by phosphatidylserine externalization, nuclear condensation, and DNA nicking.
  • GzmC's potency and kinetics are comparable to granzyme B.
  • GzmC-induced death does not involve caspases, BID cleavage, or CAD activation.
  • GzmC causes rapid mitochondrial swelling and depolarization, independent of cyclosporin A.

Conclusions:

  • Murine granzyme C is a potent inducer of target cell death.
  • GzmC utilizes distinct nuclear and mitochondrial targets, differing from granzyme B's apoptotic pathway.
  • GzmC represents a novel mechanism for cytotoxic lymphocyte-mediated cell death.