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[Studies on single cell cloning of Ginkgo biloba L]
1Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
Objective:
To investigate the relations of the productivity stability of to the homogenization of Ginkgo biloba cells by single cell clones.
Method:
Using plant single cell plate culture technique.
Results:
Adding 500 ml.L-1L-glutamine in the medium increased greatly the cell plating efficiency (PE), and within the range 0.5-5.0 x 10(4) Cell.ml-1 of the initial cell density, the PE increased with the increment of the cell density. Of the 48 cell clones obtained, the GKB content in G-22 clone reached 0.099%; and most of the clones remain stable in growth and GKB production during subcultures.
Conclusion:
During the cell and tissue culture of G. biloba, the application of plant single cell cloning technique helps to a certain extent solve the problem of productivity instability of ginkgolide.