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Updated: Jul 29, 2026

Observing and Quantifying Fibroblast-mediated Fibrin Gel Compaction
Published on: January 16, 2014
[The experimental study of intracellular actin in the scar fibroblasts]
1Department of Plastic Surgery, Ninth People's Hospital, Shanghai Second Medical University, Shanghai, P.R China 200011.
Objective:
To study the relationship between intracellular actin and scar contracture.
Methods:
Fibroblasts from 10 cases of hypertrophic scar and 5 cases of keloid were cultured in vitro. Total actin, filamentous actin(F actin), globular actin (G actin) and the ratio of F to G actin(F/G) were measured by densitometry after differential extraction and separation by polyacrylamide gel electrophoresis in the presence of sodium sulfate.
Results:
Total actin, F actin, G actin and F/G in hypertrophic scar fibroblasts were 2.38 ng/10(4) cells, 0.98 ng/10(4) cells, 1.42 ng/10(4) cells and 0.68 respectively, while in keloid fibroblasts were 1.68 ng/10(4) cells. 0.46 ng/10(4) cells, 1.26 ng/10(4) cells, and 0.36 respectively. There was significant differences between two tissues fibroblasts in the items of total actin, F actin, G actin, and F/G (P < 0.01), while no significant difference in G actin (P > 0.05).
Conclusion:
Total intracellular actin, F actin, and F/G may play an important role in the scar contracture. The hypertrophic scar and keloid can be distinguished by the contents of total intracellular actin, F actin and F/G.
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