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Updated: Sep 14, 2026

Isolation of Sertoli Cells and Peritubular Cells from Rat Testes
Published on: February 8, 2016
[Study of apoptosis in the male rat spermatogenic cells after vasostomy]
1Department of Urological Surgery, First University Hospital, West China University of Medical Sciences, Chengdu Sichuan, P. R. China 610041.
Objective:
To investigate the effect of vasostomy on apoptosis in the male rat spermatogenic cells after vasoligation.
Methods:
Model of vasoligation and vasostomy in male rat was established, and then terminal deoxynucleotidyl transferase-mediated dUTP nick labelling technique to detect the apoptosis of spermatogenic cells at 4, 8, 12, 16 weeks after vasostomy.
Results:
The number of apoptotic cells in vasostomy group was significantly lower than that of vasoligation group since 8 weeks after vasostomy(P < 0.05). The number of apoptotic cells in 8 and 12 weeks after vasostomy were significantly higher than that in prevasoligation(P < 0.05). 16 weeks after vasostomy, the number of apoptotic cells restored to the level same as that in prevasoligation stage.
Conclusion:
Vasostomy can reverse the apoptosis of spermatogenic cells due to vasoligation.
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