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[The influence of basic fibroblast growth factor on the adhesion characteristics of osteoblasts in rabbit]
1Department of Traumatology and Orthopedics, Nanfang Hospital, 1st Military Medical University, Guangzhou Guangdong, P. R. China, 510515.
Objective:
To investigate the influence of basic fibroblast growth factor (bFGF) on adhesion characteristics of osteoblasts, aimed at the important problem in bone tissue engineering of how to promote the adherence of osteoblasts to extracellular matrix materials.
Methods:
5 ng/ml, 10 ng/ml, 50 ng/ml, 100 ng/ml, 200 ng/ml bFGF were used to induce bone marrow stromal-derived osteoblasts of rabbit for 24 hours before incubation, and the common culture medium as the control. The attached cells were calculated with stereology method at 0.5 hour, 1st hour, 2nd hour, 4th hour, 8th hour after seeding.
Results:
The number of attached cells was significant higher in the experimental group when induced by 10 ng/ml bFGF than that in the control group (P < 0.01); the number did not increase with the increase of bFGF concentration and there was no significant difference between the experimental group induced by 100 ng/ml bFGF and control group, and the number was even obviously lower in the experimental group when induced by 200 ng/ml than the control group (P < 0.01).
Conclusion:
bFGF can influence the adhesion characteristics of osteoblasts, 10 ng/ml bFGF can promote the adherence of osteoblasts to matrix materials, but 200 ng/ml bFGF may inhibit cell adhesion.