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Monoclonal antibody to shiga toxin 1, which blocks receptor binding and neutralizes cytotoxicity
Hiroshi Nakao1, Chiwa Kataoka, Nobutaka Kiyokawa
1Department of Infectious Diseases, National Research Institute for Child Health and Development, Setagaya-ku, Tokyo 154-8567, Japan. hnakao@nch.go.jp
Microbiology and Immunology
|January 9, 2003
Abstract:
A monoclonal antibody, 5-5B, which neutralizes Shiga toxin 1 (Stx1) cytotoxicity of Escherichia coli, was constructed. An epitope analysis indicated that Asn55 in Stx1 B subunit was an important residue. This result and our previous results using an anti-Stx2 monoclonal antibody indicate that the region around the cysteine residue of the disulfide bond might be important for the neutralization of Stx cytotoxicity, making it a potential vaccination candidate.