Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mucin gene expression in gallbladder epithelium.

King-Teh Lee1, Tsan-Shium Liu

  • 1Department of Digestive and General Surgery, Faculty of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.

Journal of the Formosan Medical Association = Taiwan Yi Zhi
|January 9, 2003
PubMed
Summary

Gallstone formation involves altered mucin gene expression. This study found specific mucin changes in gallbladders with cholesterol or calcium bilirubinate stones, indicating their role in gallstone development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Delayed open-heart operation to remove migrated intracardiac inferior vena cava stents in a living donor liver transplantation recipient: a case report.

Nagoya journal of medical science·2025
Same author

Clinical Outcomes and Cost-Effectiveness of Osteoporosis Screening With Dual-Energy X-ray Absorptiometry.

Korean journal of radiology·2023
Same author

Inverse Probability of Treatment Weighting in 5-Year Quality-of-Life Comparison among Three Surgical Procedures for Hepatocellular Carcinoma.

Cancers·2023
Same author

Elevated intramuscular adipose tissue content with a high Ishak fibrosis stage (>3) had a negative effect on liver regeneration in cirrhotic patients undergoing portal vein embolization.

The Kaohsiung journal of medical sciences·2022
Same author

Distal migration of biliary plastic stent with duodenal perforation in liver transplantation recipients: First case report in Taiwan.

Asian journal of surgery·2022
Same author

The experience of use of percutaneous transhepatic biliary drainages for early biliary complications after liver transplantation.

The Kaohsiung journal of medical sciences·2022

Area of Science:

  • Gastroenterology
  • Molecular Biology
  • Pathology

Background:

  • Gallstone formation is linked to interactions between mucus, calcium, and lipids.
  • Gallbladder epithelium exhibits increased mucin content in the presence of gallstones.
  • Altered mucin gene expression is observed in gallbladders with black pigment stones.

Purpose of the Study:

  • To investigate mucin gene expression in gallbladders associated with cholesterol stones.
  • To evaluate mucin gene expression in gallbladders with calcium bilirubinate stones.
  • To compare mucin gene expression in gallbladders with and without stones.

Main Methods:

  • In situ hybridization was used to analyze mucin gene expression.
  • Paraffin-embedded gallbladder epithelial tissues were examined.

Related Experiment Videos

  • Expression of MUC1, MUC2, MUC3, MUC4, MUC5B, and MUC6 was assessed in three groups: cholesterol stones, calcium bilirubinate stones, and no stones.

    • Main Results:

    • MUC3, MUC5B, and MUC6 mRNA were expressed in most gallbladders, with stronger expression in those containing stones.
    • MUC2 and MUC4 were absent in stone-free gallbladders but present in those with cholesterol or calcium bilirubinate stones.
    • Increased MUC1 expression was observed in gallbladders with both types of stones compared to those without stones.

    Conclusions:

    • Gallbladders with cholesterol stones and calcium bilirubinate stones show altered mucin gene expression.
    • The presence of MUC2 and MUC4, along with increased MUC1, MUC3, MUC5B, and MUC6 expression, is indicative of these alterations.
    • These findings highlight the role of specific mucin genes in the pathogenesis of cholesterol and calcium bilirubinate gallstones.